Proteins For Sale

Miniplasminogen and Microplasminogen

Breakdown of a fibrin clot into soluble components depends on plasmin (Plm), a serine protease that is derived from the freely circulating zymogenic proenzyme plasminogen (Plg). The activated Plg is transformed into two separate subunits interconnected by two disulfide bridges. The A chain of the Plm molecule consists of 5 triple-loop disulfide kringle (Kr) domains (approximately 78-80 amino acids each), while the B chain contains a "linker" region of 20 amino acids and a serine protease domain (approximately 228 amino acids) .

Through laboratory manipulations, we have successfully created two des-kringle variants of plasminogen with potential pharmacological applications, miniplasminogen (mini-Plg) and microplasminogen (µPlg). Mini-Plg consists of only kringle 5, the linker, and the serine protease domain, while µPlg consists of only of the linker and serine protease domain itself. µPlg and mini-Plg can also be activated to microplasmin (µPlm) or miniplasmin (mini-Plm) respectively, by digestion at the peptide bond between R561 and V562 by tPA or uPA , again forming two separate subunits interconnected by the same two disulfide bridges.

µPlg and mini-Plg are potentially more potent and fast acting than other thrombolytic therapies. Moreover, they can eliminate the risk of life-threatening intracranial hemorrhage usually associated with plasminogen activators.

For pricing and other information, please contact zhu at proteomtech-inc.com.

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